Western blot analysis of extracts of various cell lines, using DSP antibody at 1:1000 dilution. Secondary antibody:HRP Goat Anti Rabbit IgG (H+L) at 1:10000 dilution. Lysates/ proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffinembedded human liver damage using DSP antibody at dilution of 1:100 (40x lens).
