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CaMKⅡ Antibody
RMA
Catalog Number： E8ET1608-47
Amount： 100ul
Applications：
WB
IHC
IF
 产品类型： Unconjugated
发货周期： 现货
说明书：     PDF
总价格：    2250元
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  • 综合
    Western blot analysis of CaMKⅡ on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY-5Y cell lysate; Lane 2: PC-12 cell lysate; Lane 3: SHG-44 cell lysate.
    ICC staining of CaMKⅡ in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
    Immunohistochemical analysis of paraffin- embedded rat brain tissue using anti-CaMKⅡ antibody. Counter stained with hematoxylin.

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