Western blot analysis of extracts of HepG2 cells, using DSP antibody at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human gastric cancer using DSP antibody at dilution of 1:100 (40x lens).
